diff --git a/gtk/theme/Adwaita/_common.scss b/gtk/theme/Adwaita/_common.scss
index 59d8a391e9..f313e8f5a9 100644
--- a/gtk/theme/Adwaita/_common.scss
+++ b/gtk/theme/Adwaita/_common.scss
@@ -1111,7 +1111,6 @@ toolbar {
   &.osd.bottom { border-radius: 0; } // positional classes for `attached` osd toolbars
 
   // toolbar separators
-  separator { background: none; }
   &.horizontal separator { margin: 0 6px; }
   &.vertical separator { margin: 6px 0; }
 }
diff --git a/gtk/theme/Adwaita/gtk-contained-dark.css b/gtk/theme/Adwaita/gtk-contained-dark.css
index afbd7b2fe2..dcefa8fcf3 100644
--- a/gtk/theme/Adwaita/gtk-contained-dark.css
+++ b/gtk/theme/Adwaita/gtk-contained-dark.css
@@ -1565,9 +1565,6 @@ toolbar, .inline-toolbar, searchbar,
   .osd.top.location-bar, toolbar.osd.bottom, .osd.bottom.inline-toolbar, searchbar.osd.bottom,
   .osd.bottom.location-bar {
     border-radius: 0; }
-  toolbar separator, .inline-toolbar separator, searchbar separator,
-  .location-bar separator {
-    background: none; }
   toolbar.horizontal separator, .horizontal.inline-toolbar separator, searchbar.horizontal separator,
   .horizontal.location-bar separator {
     margin: 0 6px; }
diff --git a/gtk/theme/Adwaita/gtk-contained.css b/gtk/theme/Adwaita/gtk-contained.css
index e7e81dc7d4..521b87945b 100644
--- a/gtk/theme/Adwaita/gtk-contained.css
+++ b/gtk/theme/Adwaita/gtk-contained.css
@@ -1569,9 +1569,6 @@ toolbar, .inline-toolbar, searchbar,
   .osd.top.location-bar, toolbar.osd.bottom, .osd.bottom.inline-toolbar, searchbar.osd.bottom,
   .osd.bottom.location-bar {
     border-radius: 0; }
-  toolbar separator, .inline-toolbar separator, searchbar separator,
-  .location-bar separator {
-    background: none; }
   toolbar.horizontal separator, .horizontal.inline-toolbar separator, searchbar.horizontal separator,
   .horizontal.location-bar separator {
     margin: 0 6px; }